Using fusions with luxAB from Vibrio harveyi MAV to quantify induction and catabolite repression of the xyl operon in Staphylococcus carnosus TM300
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Using fusions with luxAB from Vibrio harveyi MAV to quantify induction and catabolite repression of the xyl operon in Staphylococcus carnosus TM300.
The luxA,B genes from the Gram-negative marine bacterium Vibrio harveyi MAV were used in Staphylococcus carnosus TM300 as a reporter system for regulated expression of xylose utilization. The luciferase genes were fused to the xyl operon from Staphylococcus xylosus C2a. Expression of bioluminescence was induced through addition of xylose and repressed in the presence of glucose. A method to qua...
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15 صفحه اولStaphy/ococcus carnosus TM300
* Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, 61 137 Brno, Czech Republic A genome map of Staphylococcus carnosus TM300, an important microorganism in the food industry and long used as a starter culture, was constructed by pulsed-f ield gel electrophoresis of DNA fragments obtained after digestion with Notl, Sfil and Apal. The size of the chromosome wa...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1993
ISSN: 0378-1097
DOI: 10.1016/0378-1097(93)90327-x